Immediate and delayed effects of a heatwave and Prorocentrum lima ((Ehrenberg) Stein 1878) bloom on the toxin accumulation, physiology, and survival of the oyster Magallana gigas (Thunberg, 1793).

Warming could facilitate the intensification of toxic algal blooms, two important stressors for marine organisms that are predicted to co-occur more frequently in the future. We investigated the immediate and delayed effects of a heatwave and a simulated bloom (3 × 106 cells L-1) of the diarrhetic shellfish toxin (DST)-producing benthic dinoflagellate Prorocentrum lima on the survival, physiology (oxygen consumption rate, condition index, immune parameters), and toxin accumulation in the Pacific rock oyster Magallana (Crassostrea) gigas. Oysters exposed to both stressors contained higher mean DST concentrations (mean ± 1 SE: 173.3 ± 19.78 µg kg-1 soft tissue) than those exposed to P. lima bloom alone (120.4 ± 20.90 µg kg-1) and exceeded the maximum permitted levels for human consumption. Exposure to individual stressors and their combination modified the physiology of M. gigas. Oysters exposed to heatwave alone had significantly higher oxygen consumption rates (0.7 ± 0.06 mg O2 h-1 g-1) than the control (0.3 ± 0.06 mg O2 h-1 g-1). However, this was not observed in oysters exposed to both heatwave and P. lima (0.5 ± 0.06 mg O2 h-1 g-1). This alteration of the metabolic response to warming in the presence of P. lima may affect the ability of rock oysters to adapt to environmental stressors (i.e., a heatwave) to ensure survival. Immunomodulation, through changes in total hemocyte count, was observed in oysters exposed to P. lima alone and in combination with warming. Individual stressors and their combination did not influence the condition index, but one mortality was recorded in oysters exposed to both stressors. The findings of this study highlight the vulnerability of rock oysters to the predicted increased frequency of heatwaves and toxic algal blooms, and the increased likelihood of shellfish containing higher than regulatory levels of DST in warming coasts.

Bulk-like emission in the visible spectrum of colloidal LiYF4:Pr nanocrystals downsized to 10 nm.

Though Pr3+ doped LiYF4 (LiYF4:Pr3+) bulk crystals are a well-known laser gain material with several radiative transitions, their nanocrystal counterparts have not been investigated with regards to these. Through downsizing to the nanoscale, novel applications are expected, especially in composite photonic devices. For example, nanocrystals in stable colloidal form with narrow size distribution are highly desirable to reduce scattering in such composites. Herein, we synthesized monodispersed LiYF4:Pr3+ nanocrystals having a size of 10 nm resulting in colorless clear stable colloidal dispersions and conducted an extensive optical characterization for the first time. We observed unexpected yet intense emission with excited state lifetimes comparable to bulk crystals in the visible spectrum through excitation at 444 nm and 479 nm. In macroscopic bulk crystals, this emission is only exploitable through excitation of a different, subjacent energy level. A comprehensive comparison to the bulk crystals provides deeper insight into the excitation mechanism and performance of these nanocrystals. The presented results pave the way for developing application-oriented LiYF4:Pr3+ nanocrystals as emitters with tailored properties for quantum optics or biomedical applications.

Microplastic ingestion in zooplankton from the Fram Strait in the Arctic.

Some of the highest microplastic concentrations in marine environments have been reported from the Fram Strait in the Arctic. This region supports a diverse ecosystem dependent on high concentrations of zooplankton at the base of the food web. Zooplankton samples were collected during research cruises using Bongo and MOCNESS nets in the boreal summers of 2018 and 2019. Using FTIR scanning spectroscopy in combination with an automated polymer identification approach, we show that all five species of Arctic zooplankton investigated had ingested microplastics. Amphipod species, found in surface waters or closely associated with sea ice, had ingested significantly more microplastic per individual (Themisto libellula: 1.8, Themisto abyssorrum: 1, Apherusa glacialis: 1) than copepod species (Calanus hyperboreus: 0.21, Calanus glacialis/finmarchicus: 0.01). The majority of microplastics ingested were below 50 µm in size, all were fragments and several different polymer types were present. We quantified microplastics in water samples collected at six of the same stations as the Calanus using an underway sampling system (inlet at 6.5 m water depth). Fragments of several polymer types and anthropogenic cellulosic fibres were present, with an average concentration of 7 microplastic particles (MP) L-1 (0-18.5 MP L-1). In comparison to the water samples, those microplastics found ingested by zooplankton were significantly smaller, highlighting that the smaller-sized microplastics were being selected for by the zooplankton. High levels of microplastic ingestion in zooplankton have been associated with negative effects on growth, development, and fecundity. As Arctic zooplankton only have a short window of biological productivity, any negative effect could have broad consequences. As global plastic consumption continues to increase and climate change continues to reduce sea ice cover, releasing ice-bound microplastics and leaving ice free areas open to exploitation, the Arctic could be exposed to further plastic pollution which could place additional strain on this fragile ecosystem.

Performance study of a high-power single-frequency fiber amplifier architecture for gravitational wave detectors.

The next generation of interferometric gravitational wave detectors will use low-noise single-frequency laser sources at 1064 nm. Fiber amplifiers are a promising design option because of high efficiency, compact design, and superior optical beam properties compared to the current generation of laser sources for gravitational wave detectors. We developed a reliable 200 W single-frequency fiber amplifier architecture to meet the application requirements regarding relative power noise, relative pointing noise, frequency noise, linear polarization, and beam quality. We characterized several of these amplifiers and discuss performance variations resulting from manufacturing tolerances and variations in amplifier architecture. This study serves as a baseline for further power scaling via e.g., coherent beam combining experiments.

Bioavailability of Microplastics to Marine Zooplankton: Effect of Shape and Infochemicals.

The underlying mechanisms that influence microplastic ingestion in marine zooplankton remain poorly understood. Here, we investigate how microplastics of a variety of shapes (bead, fiber, and fragment), in combination with the algal-derived infochemicals dimethyl sulfide (DMS) and dimethylsulfoniopropionate (DMSP), affect the ingestion rate of microplastics in three species of zooplankton, the copepods Calanus helgolandicus and Acartia tonsa and larvae of the European lobster Homarus gammarus. We show that shape affects microplastic bioavailability to different species of zooplankton, with each species ingesting significantly more of a certain shape: C. helgolandicus-fragments (P < 0.05); A. tonsa-fibers (P < 0.01); H. gammarus larvae-beads (P < 0.05). Thus, different feeding strategies between species may affect shape selectivity. Our results also showed significantly increased ingestion rates by C. helgolandicus on all microplastics that were infused with DMS (P < 0.01) and by H. gammarus larvae and A. tonsa on DMS-infused fibers and fragments (P < 0.05). By using a range of more environmentally relevant microplastics, our findings highlight how the feeding strategies of different zooplankton species may influence their susceptibility to microplastic ingestion. Furthermore, our novel study suggests that species reliant on chemosensory cues to locate their prey may be at an increased risk of ingesting aged microplastics in the marine environment.

Phytoplankton-derived zwitterionic gonyol and dimethylsulfonioacetate interfere with microbial dimethylsulfoniopropionate sulfur cycling.

The marine sulfur cycle is substantially fueled by the phytoplankton osmolyte dimethylsulfoniopropionate (DMSP). This metabolite can be metabolized by bacteria, which results in the emission of the volatile sulfur species methanethiol (MeSH) and the climate-cooling dimethylsulfide (DMS). It is generally accepted that bacteria contribute significantly to DMSP turnover. We show that the other low molecular weight zwitterionic dimethylsulfonio compounds dimethylsulfonioacetate (DMSA) and gonyol are also widely distributed in phytoplankton and can serve as alternative substrates for volatile production. DMSA was found in 11 of the 16 surveyed phytoplankton species, and gonyol was detected in all haptophytes and dinoflagellates. These prevalent zwitterions are also metabolized by marine bacteria. The patterns of bacterial MeSH and DMS release were dependent on the zwitterions present. Certain bacteria metabolize DMSA and gonyol and release MeSH, in others gonyol inhibited DMS-producing enzymes. If added in addition to DMSP, gonyol entirely inhibited the formation of volatiles in Ruegeria pomeroyi. In contrast, no substantial effect of this compound was observed in the DMSP metabolism of Halomonas sp. We argue that the production of DMSA and gonyol and their inhibitory properties on the release of volatiles from DMSP has the potential to modulate planktonic sulfur cycling between species.

Single-frequency chirally coupled-core all-fiber amplifier with 100 W in a linearly polarized TEM00 mode.

Large mode area fibers have become indispensable in addressing the power requirements of laser sources in gravitational wave detectors. Besides high power capabilities, the system must provide an excellent beam quality and polarization. In this Letter, we present the characterization of a monolithic high-power fiber amplifier at 1064 nm, built using an ytterbium-doped chirally coupled-core fiber, which achieves an output power of 100 W in a linearly polarized $ {{\rm TEM}_{00}} $TEM00 mode in an all-fiber setup.

High power, single-frequency, monolithic fiber amplifier for the next generation of gravitational wave detectors.

Low noise, high power single-frequency lasers and amplifiers are key components of interferometric gravitational wave detectors. One way to increase the detector sensitivity is to increase the power injected into the interferometers. We developed a fiber amplifier engineering prototype with a pump power limited output power of 200 W at 1064 nm. No signs of stimulated Brillouin scattering are observed at 200 W. At the maximum output power the polarization extinction ratio is above 19 dB and the fractional power in the fundamental transverse mode (TEM 00) was measured to be 94.8 %. In addition, measurements of the frequency noise, relative power noise, and relative pointing noise were performed and demonstrate excellent low noise properties over the entire output power slope. In the context of single-frequency fiber amplifiers, the measured relative pointing noise below 100 Hz and the higher order mode content is, to the best of our knowledge, at 200 W the lowest ever measured. A long-term test of more than 695 h demonstrated stable operation without beam quality degradation. It is also the longest single-frequency fiber amplifier operation at 200 W ever reported.

Grating assisted glass fiber coupler for mode selective co-directional coupling.

To keep pace with the increasing demand of transmission capacity, space division multiplexing technologies are currently intensively investigated. In this context, mode selective glass fiber couplers are of great interest due to their compatibility with existing glass fiber networks. In this work, we present a novel type of mode selective glass fiber coupler for co-directional coupling based on fiber gratings and fused asymmetric fibers. The achieved mode selective coupling efficiency agrees well with numerical simulations performed for comparison. The benefits of the grating approach are a lower mode crosstalk and a simple adaption of the propagation constants through changing of the grating-period.

Bioavailability and effects of microplastics on marine zooplankton: A review.

Microplastics are abundant and widespread in the marine environment. They are a contaminant of global environmental and economic concern. Due to their small size a wide range of marine species, including zooplankton can ingest them. Research has shown that microplastics are readily ingested by several zooplankton taxa, with associated negative impacts on biological processes. Zooplankton is a crucial food source for many secondary consumers, consequently this represents a route whereby microplastic could enter the food web and transfer up the trophic levels. In this review we aim to: 1) evaluate the current knowledge base regarding microplastic ingestion by zooplankton in both the laboratory and the field; and 2) summarise the factors which contribute to the bioavailability of microplastics to zooplankton. Current literature shows that microplastic ingestion has been recorded in 39 zooplankton species from 28 taxonomic orders including holo- and meroplanktonic species. The majority of studies occurred under laboratory conditions and negative effects were reported in ten studies (45%) demonstrating effects on feeding behaviour, growth, development, reproduction and lifespan. In contrast, three studies (14%) reported no negative effects from microplastic ingestion. Several physical and biological factors can influence the bioavailability of microplastics to zooplankton, such as size, shape, age and abundance. We identified that microplastics used in experiments are often different to those quantified in the marine environment, particularly in terms of concentration, shape, type and age. We therefore suggest that future research should include microplastics that are more representative of those found in the marine environment at relevant concentrations. Additionally, investigating the effects of microplastic ingestion on a broader range of zooplankton species and life stages, will help to answer key knowledge gaps regarding the effect of microplastic on recruitment, species populations and ultimately broader economic consequences such as impacts on shell- and finfish stocks.

Pump wavelength dependence of ASE and SBS in single-frequency EYDFAs.

In this Letter, the pump wavelength dependence of the amplified spontaneous emission (ASE) and the threshold of stimulated Brillouin scattering (SBS) in a typical single-frequency continuous wave Er3+:Yb3+-codoped fiber amplifier is investigated numerically. The Er3+:Yb3+ system is modeled as coupled two- and three-level systems, linked by a Förster resonance energy transfer process and described by the corresponding rate equations. The evolution of the pump and signal power along the fiber is modeled by differential equations, taking into account the steady-state population densities. Since the absorption at 976 nm can exceed the Yb3+-to-Er3+ energy transfer rate in high-power operation, unsaturated gain at around 1.0 µm can generate excessive ASE. Off-peak pumping with commercially available pump diodes at 915 or 940 nm spatially distributes the energy over a longer distance. For the studied amplifier configuration, energy transfer bottlenecking is prevented without the onset of SBS.

Microstructured fiber cladding light stripper for kilowatt-class laser systems.

An all-glass microstructured high-power cladding mode stripper capable of handling cladding light of up to a power of approximately 350 W with stripping efficiencies >22 dB is presented. An optimized graded structure pattern increased the device's reliability and its power-handling capabilities. Subjected to a 500 h stress test, the device shows no degradation.

All-fiber, single-frequency, and single-mode Er3+:Yb3+ fiber amplifier at 1556 nm core-pumped at 1018 nm.

Emerging applications, such as gravitational wave astronomy, demand single-frequency lasers with diffraction-limited emission at 1.5 µm. Fiber amplifiers have greatly evolved to fulfill these requirements. Hundreds of watts are feasible using large-mode-area and specialty fibers. However, their application in a few watts to tens of watts in monolithic systems is unnecessarily complex due to the poor commercial availability of fiber components and standard integration procedures. In this Letter we propose and experimentally demonstrate a novel and simple method to amplify single-frequency signals at 1.5 µm up to tens of watts by core-pumping single-mode Er3+:Yb3+ fiber amplifiers at 1018 nm. The proof-of-principle system is tested with different active fibers, lengths, and seed power levels. Over 11 W with an efficiency of more than 48% versus launched power is achieved. Additionally, performance degradation during operation was observed for which photodarkening due to P1 defects might be an explanation.

Long-Period Gratings in Highly Germanium-Doped, Single-Mode Optical Fibers for Sensing Applications.

Long-period fiber gratings (LPGs) are well known for their sensitivity to external influences, which make them interesting for a large number of sensing applications. For these applications, fibers with a high numerical aperture (i.e., fibers with highly germanium (Ge)-doped fused silica fiber cores) are more attractive since they are intrinsically photosensitive, as well as less sensitive to bend- and microbend-induced light attenuations. In this work, we introduce a novel method to inscribe LPGs into highly Ge-doped, single-mode fibers. By tapering the optical fiber, and thus, tailoring the effective indices of the core and cladding modes, for the first time, an LPG was inscribed into such fibers using the amplitude mask technique and a KrF excimer laser. Based on this novel method, sensitive LPG-based fiber optic sensors only a few millimeters in length can be incorporated in bend-insensitive fibers for use in various monitoring applications. Moreover, by applying the described inscription method, the LPG spectrum can be influenced and tailored according to the specific demands of a particular application.

Flux of the biogenic volatiles isoprene and dimethyl sulfide from an oligotrophic lake.

Biogenic volatile organic compounds (BVOCs) affect atmospheric chemistry, climate and regional air quality in terrestrial and marine atmospheres. Although isoprene is a major BVOC produced in vascular plants, and marine phototrophs release dimethyl sulfide (DMS), lakes have been widely ignored for their production. Here we demonstrate that oligotrophic Lake Constance, a model for north temperate deep lakes, emits both volatiles to the atmosphere. Depth profiles indicated that highest concentrations of isoprene and DMS were associated with the chlorophyll maximum, suggesting that their production is closely linked to phototrophic processes. Significant correlations of the concentration patterns with taxon-specific fluorescence data, and measurements from algal cultures confirmed the phototrophic production of isoprene and DMS. Diurnal fluctuations in lake isoprene suggested an unrecognised physiological role in environmental acclimation similar to the antioxidant function of isoprene that has been suggested for marine biota. Flux estimations demonstrated that lakes are a currently undocumented source of DMS and isoprene to the atmosphere. Lakes may be of increasing importance for their contribution of isoprene and DMS to the atmosphere in the arctic zone where lake area coverage is high but terrestrial sources of BVOCs are small.

Single-frequency fiber amplifier at 1.5 µm with 100 W in the linearly-polarized TEM00 mode for next-generation gravitational wave detectors.

Next-generation gravitational wave detectors require single-frequency and high power lasers at a wavelength of 1.5 µm addressing a set of demanding requirements such as linearly-polarized TEM00 radiation with low noise to run for long periods. In this context, fiber amplifiers in MOPA configuration are promising candidates to fulfill these requirements. We present a single-frequency monolithic Er:Yb co-doped fiber amplifier (EYDFA) at 1.5 µm with a linearly-polarized TEM00 output power of 100 W. The EYDFA is pumped off-resonant at 940 nm to enhance the Yb-to-Er energy transfer efficiency and enable higher ASE threshold. We also performed numerical simulations to investigate the off-resonant pumping scheme and confirm the corresponding experimental results.

Influence of the third energy level on the gain dynamics of EDFAs: analytical model and experimental validation.

We report an analytical model and experimental validation of the temporal dynamics of 3-level system fiber amplifiers. The model predictions show a good agreement with the measured pump power to output power and the pump power to output phase transfer functions in an EDFA pumped at 976 nm, as well as with the typical literature values for the spontaneous lifetime of the involved energy levels. The measurements show a linear relation between the effective lifetime of the meta-stable level and the output power, and a filtering of the temperature-induced phase-shift due to the quantum defect at a sufficiently high frequency modulation.

Air exposure of coral is a significant source of dimethylsulfide (DMS) to the atmosphere.

Corals are prolific producers of dimethylsulfoniopropionate (DMSP). High atmospheric concentrations of the DMSP breakdown product dimethylsulfide (DMS) have been linked to coral reefs during low tides. DMS is a potentially key sulfur source to the tropical atmosphere, but DMS emission from corals during tidal exposure is not well quantified. Here we show that gas phase DMS concentrations (DMSgas) increased by an order of magnitude when three Indo-Pacific corals were exposed to air in laboratory experiments. Upon re-submersion, an additional rapid rise in DMSgas was observed, reflecting increased production by the coral and/or dissolution of DMS-rich mucus formed by the coral during air exposure. Depletion in DMS following re-submersion was likely due to biologically-driven conversion of DMS to dimethylsulfoxide (DMSO). Fast Repetition Rate fluorometry showed downregulated photosynthesis during air exposure but rapid recovery upon re-submersion, suggesting that DMS enhances coral tolerance to oxidative stress during a process that can induce photoinhibition. We estimate that DMS emission from exposed coral reefs may be comparable in magnitude to emissions from other marine DMS hotspots. Coral DMS emission likely comprises a regular and significant source of sulfur to the tropical marine atmosphere, which is currently unrecognised in global DMS emission estimates and Earth System Models.

The ocean sampling day consortium.

Ocean Sampling Day was initiated by the EU-funded Micro B3 (Marine Microbial Biodiversity, Bioinformatics, Biotechnology) project to obtain a snapshot of the marine microbial biodiversity and function of the world's oceans. It is a simultaneous global mega-sequencing campaign aiming to generate the largest standardized microbial data set in a single day. This will be achievable only through the coordinated efforts of an Ocean Sampling Day Consortium, supportive partnerships and networks between sites. This commentary outlines the establishment, function and aims of the Consortium and describes our vision for a sustainable study of marine microbial communities and their embedded functional traits.

TEM00 mode content measurements on a passive leakage channel fiber.

Mode content measurements with a scanning ring cavity were performed in order to determine the TEM00 mode content of the output beam profile of a resonantly enhanced leakage channel fiber. The measurements were performed at 1.0 and 1.5 µm. In addition, the influence of different bending diameters as well as launching conditions has been investigated. Furthermore, a numerical simulation was used to determine the maximum theoretical TEM00 overlap, if only the fundamental fiber mode is guided. The simulation was also used to analyze how the TEM00 overlap for the case of any additional higher order fiber mode can be determined consistently.